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γ h2ax immunofluorescence  (Beyotime)


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    Beyotime γ h2ax immunofluorescence
    γ H2ax Immunofluorescence, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B3+h2ax+immunofluorescence/DNA+Damage+Assay+Kit+by+%CE%B3-H2AX+Immunofluorescence/pmc13093934-76-7-9
    Average 99 stars, based on 282 article reviews
    γ h2ax immunofluorescence - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: WTAP Contributes to Periodontitis Pathogenesis by Promoting PDLSC Senescence and Impairing Osteogenic Differentiation via m6A-Dependent Regulation of TP53BP1.
    Article Snippet: Images were acquired under the microscope (Keyence) and quantified with Image J 1.8.0 software. .. All steps were conducted employing the DNA Damage Assay Kit by γ‐H2AX Immunofluorescence (C2035, Beyotime). .. Based on the manual of the Senescence β‐Galactosidase Staining Kit (C0602, Beyotime), the senescence of cells was measured.

    Article Title: WTAP Contributes to Periodontitis Pathogenesis by Promoting PDLSC Senescence and Impairing Osteogenic Differentiation via m6A‐Dependent Regulation of TP53BP1
    Article Snippet: Images were acquired under the microscope (Keyence) and quantified with Image J 1.8.0 software. .. All steps were conducted employing the DNA Damage Assay Kit by γ‐H2AX Immunofluorescence (C2035, Beyotime). .. Based on the manual of the Senescence β‐Galactosidase Staining Kit (C0602, Beyotime), the senescence of cells was measured.

    Article Title: Dietary N-acetylcysteine enhances sperm motility by remodeling the rumen microbiome and its metabolic axis in goats.
    Article Snippet: .. DNA damage in spermatozoa was assessed using γ-H2AX immunofluorescence (Beyotime Biotechnology, C2035S) with a DNA damage assay kit. ..

    Article Title: The telomerase reverse transcriptase-derived peptide GN11 alleviates glycolysis and cellular senescence in the fibrotic kidney by inhibiting the TGF-β1/Smad3/lncRNA Oip5-os1/miR-30c-5p/Rock2 axis.
    Article Snippet: Renal fibrosis represents the ultimate pathological manifestation of chronic kidney disease (CKD).. To date, no clinically approved drugs that specifically target renal fibrosis are available.. In this study, we discovered that GV1001(GV), a peptide fragment derived from human telomerase reverse transcriptase (hTERT), can inhibit the unilateral ureteral obstruction (UUO)-induced renal fibrosis.

    Article Title: Efficient apoptosis via double-strand breaks of DNA in human epithelial cells induced by non-thermal effect of high-field broadband terahertz waves
    Article Snippet: .. DNA Damage Assays were evaluated after irradiation of the cells by the high-field THz waves using the DNA damage assay kit by γ-H2AX immunofluorescence, as per manufacturer’s instructions (Beyotime, C2035S). ..

    Article Title: Rational engineering of cholesterol-modified prodrug nanoassemblies for improving the tumor selectivity and safety of mitoxantrone
    Article Snippet: The 96-well plate, 24-well plate, 12-well plate and 6-well plate were purchased from NEST Biotechnology Co. Ltd. (Wuxi, China). .. DNA Damage Assay Kit by γ-H2AX Immunofluorescence was procured from Beyotime Biotechnology (Shanghai, China). ..

    Article Title: Dietary N-acetylcysteine enhances sperm motility by remodeling the rumen microbiome and its metabolic axis in goats
    Article Snippet: .. DNA damage in spermatozoa was assessed using γ-H2AX immunofluorescence (Beyotime Biotechnology, C2035S) with a DNA damage assay kit. ..

    Article Title: 131 I/MTX-loaded engineered outer membrane vesicles for sequentially coordinated radio-metabolic-immunotherapy of glioma.
    Article Snippet: .. BCA Protein Assay Kit (Cat# P0012), 3,3’-dioctadecyloxacarbocyanine perchlorate (DiI) (Cat# C1036), Cell Counting Kit-8 (CCK8) (Cat# C0038), Annexin V-FITC Apoptosis Detection Kit (Cat# C1062S) and DNA Damage Assay Kit by γ-H2AX Immunofluorescence (Cat# C2037S) were obtained from Beyotime Biotechnology (Shanghai, China). .. Na125I and Na131I solutions were procured from Shanghai Xinke Pharmaceutical Co., Ltd. (Shanghai, China).

    Expressing:

    Article Title: The telomerase reverse transcriptase-derived peptide GN11 alleviates glycolysis and cellular senescence in the fibrotic kidney by inhibiting the TGF-β1/Smad3/lncRNA Oip5-os1/miR-30c-5p/Rock2 axis.
    Article Snippet: Renal fibrosis represents the ultimate pathological manifestation of chronic kidney disease (CKD).. To date, no clinically approved drugs that specifically target renal fibrosis are available.. In this study, we discovered that GV1001(GV), a peptide fragment derived from human telomerase reverse transcriptase (hTERT), can inhibit the unilateral ureteral obstruction (UUO)-induced renal fibrosis.

    Irradiation:

    Article Title: Efficient apoptosis via double-strand breaks of DNA in human epithelial cells induced by non-thermal effect of high-field broadband terahertz waves
    Article Snippet: .. DNA Damage Assays were evaluated after irradiation of the cells by the high-field THz waves using the DNA damage assay kit by γ-H2AX immunofluorescence, as per manufacturer’s instructions (Beyotime, C2035S). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: 131 I/MTX-loaded engineered outer membrane vesicles for sequentially coordinated radio-metabolic-immunotherapy of glioma.
    Article Snippet: .. BCA Protein Assay Kit (Cat# P0012), 3,3’-dioctadecyloxacarbocyanine perchlorate (DiI) (Cat# C1036), Cell Counting Kit-8 (CCK8) (Cat# C0038), Annexin V-FITC Apoptosis Detection Kit (Cat# C1062S) and DNA Damage Assay Kit by γ-H2AX Immunofluorescence (Cat# C2037S) were obtained from Beyotime Biotechnology (Shanghai, China). .. Na125I and Na131I solutions were procured from Shanghai Xinke Pharmaceutical Co., Ltd. (Shanghai, China).

    CCK-8 Assay:

    Article Title: 131 I/MTX-loaded engineered outer membrane vesicles for sequentially coordinated radio-metabolic-immunotherapy of glioma.
    Article Snippet: .. BCA Protein Assay Kit (Cat# P0012), 3,3’-dioctadecyloxacarbocyanine perchlorate (DiI) (Cat# C1036), Cell Counting Kit-8 (CCK8) (Cat# C0038), Annexin V-FITC Apoptosis Detection Kit (Cat# C1062S) and DNA Damage Assay Kit by γ-H2AX Immunofluorescence (Cat# C2037S) were obtained from Beyotime Biotechnology (Shanghai, China). .. Na125I and Na131I solutions were procured from Shanghai Xinke Pharmaceutical Co., Ltd. (Shanghai, China).



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    γ h2ax immunofluorescence  (Beyotime)
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    Beyotime γ h2ax immunofluorescence
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    γ h2ax detection kit  (Beyotime)
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    DNA damage markers induced by Pb and/or Cd in TK6 cells. ( A , C ) representative immunofluorescence staining images and quantification <t>of</t> <t>γ-H2AX</t> fluorescence intensity in TK6 cells. ( B , D , E ) representative the Comet assay images and quantitative analyses of Tail Length and Tail DNA%. Note: * p < 0.05, ** p < 0.01 for Pb and/or Cd group vs. the control group; while ## p < 0.01 for the single exposure groups vs. the mixed group; § p < 0.05, §§ p < 0.01 for the resveratrol group vs. the mixed group.
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    dna damage assay kit  (Beyotime)
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    Seq1 and Seq3 prevent senescence and senescence-associated secretory phenotype (SASP), which are nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent. (A) Representative immunofluorescence (IF) staining images of positive cells of γ-H2AX (green) and 4,6-diamidino-2-phenylindole (DAPI) in <t>DNA</t> <t>damage</t> assay following knockdown of Nrf2 or knockdown of Kelch-like ECH-associated protein 1 (Keap1). NC: negative control. (B) Quantitation of the mean number of γ-H2AX foci/cell upon Nrf2 knockdown. (C) Quantitation of the mean number of γ-H2AX foci/cell upon Keap1 knockdown. (D) Representative IF staining images of positive cells of reactive oxygen species (ROS) (green) and DAPI in determination of ROS following knockdown of Nrf2 and knockdown of Keap1. (E) Fluorescence intensity of ROS upon Nrf2 knockdown. (F) Fluorescence intensity of ROS upon Keap1 knockdown. (G) Quantification of messenger RNA (mRNA) expression for senescence secretory phenotype in human keratinocytes (HaCat) following knockdown of Nrf2 24 h after radiation. (H) Quantification of mRNA expression for senescence secretory phenotype in HaCat following knockdown of Keap1 24 h after radiation. All data are presented as means ± standard deviation (SD) ( n = 3). Statistical significance is denoted by ∗∗∗ P < 0.001 and ∗∗∗∗ P < 0.0001 vs. ultraviolet A (UVA)-irradiated group.
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    Image Search Results


    DNA damage markers induced by Pb and/or Cd in TK6 cells. ( A , C ) representative immunofluorescence staining images and quantification of γ-H2AX fluorescence intensity in TK6 cells. ( B , D , E ) representative the Comet assay images and quantitative analyses of Tail Length and Tail DNA%. Note: * p < 0.05, ** p < 0.01 for Pb and/or Cd group vs. the control group; while ## p < 0.01 for the single exposure groups vs. the mixed group; § p < 0.05, §§ p < 0.01 for the resveratrol group vs. the mixed group.

    Journal: Toxics

    Article Title: Oxidative Stress, DNA Damage, DNA Repair Inhibition, and Apoptosis Induced by Lead and Cadmium Combined Exposure in TK6 Cells

    doi: 10.3390/toxics14040341

    Figure Lengend Snippet: DNA damage markers induced by Pb and/or Cd in TK6 cells. ( A , C ) representative immunofluorescence staining images and quantification of γ-H2AX fluorescence intensity in TK6 cells. ( B , D , E ) representative the Comet assay images and quantitative analyses of Tail Length and Tail DNA%. Note: * p < 0.05, ** p < 0.01 for Pb and/or Cd group vs. the control group; while ## p < 0.01 for the single exposure groups vs. the mixed group; § p < 0.05, §§ p < 0.01 for the resveratrol group vs. the mixed group.

    Article Snippet: DNA damage was further assessed using a γ-H2AX detection kit (Beyotime, Shanghai, China; C2035S), which quantifies γ-H2AX levels, a well-established biomarker for DNA double-strand breaks.

    Techniques: Immunofluorescence, Staining, Fluorescence, Single Cell Gel Electrophoresis, Control

    Seq1 and Seq3 prevent senescence and senescence-associated secretory phenotype (SASP), which are nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent. (A) Representative immunofluorescence (IF) staining images of positive cells of γ-H2AX (green) and 4,6-diamidino-2-phenylindole (DAPI) in DNA damage assay following knockdown of Nrf2 or knockdown of Kelch-like ECH-associated protein 1 (Keap1). NC: negative control. (B) Quantitation of the mean number of γ-H2AX foci/cell upon Nrf2 knockdown. (C) Quantitation of the mean number of γ-H2AX foci/cell upon Keap1 knockdown. (D) Representative IF staining images of positive cells of reactive oxygen species (ROS) (green) and DAPI in determination of ROS following knockdown of Nrf2 and knockdown of Keap1. (E) Fluorescence intensity of ROS upon Nrf2 knockdown. (F) Fluorescence intensity of ROS upon Keap1 knockdown. (G) Quantification of messenger RNA (mRNA) expression for senescence secretory phenotype in human keratinocytes (HaCat) following knockdown of Nrf2 24 h after radiation. (H) Quantification of mRNA expression for senescence secretory phenotype in HaCat following knockdown of Keap1 24 h after radiation. All data are presented as means ± standard deviation (SD) ( n = 3). Statistical significance is denoted by ∗∗∗ P < 0.001 and ∗∗∗∗ P < 0.0001 vs. ultraviolet A (UVA)-irradiated group.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Novel bioactive peptides targeting Keap1-Nrf2 interaction for combating UVA-induced skin aging: Computational discovery and experimental validation

    doi: 10.1016/j.jpha.2025.101446

    Figure Lengend Snippet: Seq1 and Seq3 prevent senescence and senescence-associated secretory phenotype (SASP), which are nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent. (A) Representative immunofluorescence (IF) staining images of positive cells of γ-H2AX (green) and 4,6-diamidino-2-phenylindole (DAPI) in DNA damage assay following knockdown of Nrf2 or knockdown of Kelch-like ECH-associated protein 1 (Keap1). NC: negative control. (B) Quantitation of the mean number of γ-H2AX foci/cell upon Nrf2 knockdown. (C) Quantitation of the mean number of γ-H2AX foci/cell upon Keap1 knockdown. (D) Representative IF staining images of positive cells of reactive oxygen species (ROS) (green) and DAPI in determination of ROS following knockdown of Nrf2 and knockdown of Keap1. (E) Fluorescence intensity of ROS upon Nrf2 knockdown. (F) Fluorescence intensity of ROS upon Keap1 knockdown. (G) Quantification of messenger RNA (mRNA) expression for senescence secretory phenotype in human keratinocytes (HaCat) following knockdown of Nrf2 24 h after radiation. (H) Quantification of mRNA expression for senescence secretory phenotype in HaCat following knockdown of Keap1 24 h after radiation. All data are presented as means ± standard deviation (SD) ( n = 3). Statistical significance is denoted by ∗∗∗ P < 0.001 and ∗∗∗∗ P < 0.0001 vs. ultraviolet A (UVA)-irradiated group.

    Article Snippet: IF detection of γ-H2AX was performed using the DNA Damage Assay Kit (C2035S, Beyotime, Shanghai, China), which is specific for γ-H2AX, following the manufacturer’s protocol.

    Techniques: Immunofluorescence, Staining, Knockdown, Negative Control, Quantitation Assay, Fluorescence, Expressing, Standard Deviation, Irradiation